Facts About attune flow cytometer Revealed
Facts About attune flow cytometer Revealed
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Information-enriched characterization of mobile populations. Morphological info from images can increase to the richness of flow cytometry facts. By way of example, the determine displays an usually typical apoptosis assay applying Annexin V and PI, including mobile imaging to characterize cells in Each and every populace to expose morphologically distinctive attributes. These insights couldn't are received from multiplex staining alone.
No matter whether you’re a beginner or a professional, explore our assets to advance your investigation and knowledge in flow cytometry.
Blend acoustic concentrating technological innovation with higher-parameter Evaluation for versatile spectral unmixing and conventional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing that has a higher-velocity brightfield camera for simultaneous substantial throughput flow cytometry and higher resolution brightfield imaging.
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Here, an experienced consumer has gated singlets confidently. Soon after assessing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate consists of more than 4% aggregates.
Pair your Attune flow cytometer by having an autosampler for improved workflow performance, and incorporate robotic integration for maximum throughput.
Reliable graphic excellent even at superior throughput Acoustic focusing along with a substantial-speed digicam Mix to graphic these T cells regularly at small or large flow rates. Conveniently modify focus and camera configurations to satisfy experimental prerequisites.
The Attune NxT cytometer may be the reliable workhorse instrument your lab can count on for constant and trusted performance. The Attune CytPix design contains a superior-pace brightfield camera and AI-driven impression analysis software program, with current upgrades which include equipment Understanding instruments and an intuitive person interface, enabling Improved details precision, accuracy, and a lot quicker insights into sample biology.
Maybe most of all, these occasions have cells of Obviously various phenotypes which could lead to incorrect conclusions concerning double good gatherings (particularly in exceptional populations).
Sound waves allow exact mobile alignment, causing Outstanding knowledge top quality and ~10X a lot quicker sample flow costs.
We Beforehand shown the power of imaging Motor vehicle-T/Ramos mobile interactions. Let’s examine just the inhabitants of finest interest, the double optimistic functions, To find out more. We can now use extended morphological parameters (in this article, circularity and skewness of intensity) to even more analyze the capabilities of such populations and refine gating on these situations, raising facts robustness.
Developed-in dimensionality reduction tools increased resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to substantiate expected populace resolution in lysed full human blood. Person can watch events from Each individual cluster for visual confirmation of mobile phenotypes.
Versatile payment strategy from Agilent Economical Solutions let you receive the important instrumentation you would like for immediate project, or to improve your lab.
Gating on these extended parameters enables you to click here speedily and accurately discover populations of curiosity and make sure gating strategy with little or no guide evaluate. The Attune program offers access to point out-of-the-art equipment Studying Evaluation equipment inside an expertly made user interface, rendering it extremely user friendly.
The Attune Xenith cytometer is often a substantial-parameter spectral analyzer, furnishing equally spectral unmixing and standard flow capabilities for detailed and versatile cell Evaluation.
Agilent Benefit Assure guarantees you no less than a decade of instrument use in the day you buy. In any other case, We'll credit score you with the residual worth of the procedure toward an upgraded model.
In this article, we clearly show that by gating approaches employing graphic-primarily based quantitative parameters can distinguish interacting cells from coincident occasions to a lot more precisely review interacting cells.